Journal: PLoS ONE

Article Title: Hypothyroidism and Its Rapid Correction Alter Cardiac Remodeling

doi: 10.1371/journal.pone.0109753

Figure Lengend Snippet: Changes in the BNP ( A ) and cTnT ( B ) levels in the different groups. Data are represented as the mean ± SEM. n = 10 animals for each group. Statistical analysis was performed with Kruskal-Wallis One-way ANOVA on rank tests followed by post hoc Dunn's multiple comparison tests. * p <0.05 vs. control; # p <0.01 vs. PTU.

Article Snippet: The BNP, CRP, IL6, TNF-α, TGF-β1, adiponectin and leptin ELISA kit reagents were purchased from Abcam, Cambridge - UK, and the fT3, fT4, TSH and cTnT ELISA kit reagents were purchased from CusaBio, Hubei, China.

Techniques: Comparison, Control

Journal: eLife

Article Title: Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice

doi: 10.7554/eLife.62174

Figure Lengend Snippet: ( A ) Tissue levels of total non-heme, ferrous, and ferric iron in Ncoa4 +/+ and Ncoa4 –/– hearts 4 weeks after transverse aortic constriction (TAC) ( n = 6 biologically independent samples). The ratio of the ferrous iron level to the FTH1 protein level is shown in the right-most panel. The FTH1 protein level for sham-operated Ncoa4 +/+ estimated as Western blot analysis in hearts was set to 1. ( B ) Malondialdehyde (MDA) levels in heart homogenates ( n = 5 biologically independent samples). ( C ) Ptgs2 mRNA levels in the heart ( n = 8 biologically independent samples). ( D ) Serum troponin T (TnT) levels ( n = 8 biologically independent samples). ( E ) Glutathione peroxidase 4 (GPX4) levels in the heart homogenates. The right panel shows the ratio of GPX4 to GAPDH ( n = 6 biologically independent samples). ( F ) The levels of total glutathione (GSH+GSSG), reduced (GSH) and oxidized (GSSG) glutathione and the ratio of GSH to GSSG in heart homogenates ( n = 5 biologically independent samples). GSH levels were calculated by subtracting GSSG from total glutathione. ( G ) Cardiac glutamine levels ( n = 5 biologically independent samples). The data were evaluated by one-way analysis of variance (ANOVA), followed by Tukey–Kramer’s post hoc test. * p<0.05, ** p<0.001, *** p<0.0001. NS, p>0.05. Exact p-values are provided in . Figure 4—source data 1. Source data for . Figure 4—source data 2. Hematological parameters and red cell indices in Ncoa4 +/+ and Ncoa4 –/– mice. PCV, packed cell volume; MCV, mean corpuscular volume; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration; CH, hemoglobin concentration. Data are the mean ± SEM. n indicates the number of biologically independent samples. The data were evaluated by one-way analysis of variance (ANOVA), followed by Tukey–Kramer’s post hoc test. * p=0.0418 and ** p=0.0108 versus the corresponding sham-operated group. Exact p-values are provided in . Figure 4—source data 3. Source data for data table provided in .

Article Snippet: Commercial assay or kit , ELISA Kit for Troponin T Type 2, Cardiac (TNNT2) , Cloud-Clone , SED232Mu , .

Techniques: Western Blot, Concentration Assay

Journal: eLife

Article Title: Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice

doi: 10.7554/eLife.62174

Figure Lengend Snippet:

Article Snippet: Commercial assay or kit , ELISA Kit for Troponin T Type 2, Cardiac (TNNT2) , Cloud-Clone , SED232Mu , .

Techniques: Western Blot, Immunofluorescence, Diagnostic Assay, Magnetic Beads, SYBR Green Assay, Staining, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Iron Assay, Multiple Displacement Amplification, Glutamate Assay, Electrophoretic Mobility Shift Assay, PCR Cloning, Software

Journal: International Journal of Molecular Medicine

Article Title: Pterostilbene inhibits nutrient metabolism and induces apoptosis through AMPK activation in multiple myeloma cells

doi: 10.3892/ijmm.2018.3857

Figure Lengend Snippet: PTE is a safe drug for the treatment of MM. (A) Liver damage markers, including ALT, AST, ALP and TBIL were measured in serum samples from NOD/SCID mice treated with 5% DMSO or PTE with an automatic biochemical analyzer. (B) Kidney function markers BUN and Scr were measured in serum samples from NOD/SCID mice treated with 5% DMSO or PTE by an automatic biochemical analyzer. (C) Cardiac injury markers cTnT and CK-MB were measured with an ELISA kit. The values are expressed as means ± standard deviation. PTE, pterostilbene; MM, multiple myeloma; ALT, alanine aminotransferase; AST, aspartate amino transferase; ALP, alkaline phosphatase; TBIL, total bilirubin; DMSO, dimethyl sulfoxide; BUN, urea nitrogen; Scr, serum creatinine; cTnT, cardiac troponins T; CK-MB, creatine kinase-MB.

Article Snippet: Concentrations of CK-MB and cTnT were determined quantitatively using the commercially available CK-MB ELISA kit (cat. no. FLSW-M1025; Kehua Bio-Engineering co., Ltd., Shanghai, China) and cTnT ELISA kit (cat. no. FLSW-M763; Kehua Bio-Engineering co., Ltd.) according to the manufacturer's protocol.

Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation